Homocysteine increases the synthesis of adrenomedullin in vascular fibroblasts of rats

Homocysteine (Hcy), a highly reactive sulfur- containing amino acid, is an intermediate product of methionine metabolism. Elevation of plasma Hcy has been widely studied as an independent risk factor for many cardiovascular diseases such as atherosclerosis, thrombosis, etc.[1]. Inherited hyperhomocysteinemia cases usually suffer from occlusion or thrombosis in arteries and large veins before the age of 20. Hyperhomocysteinemia significantly reduces the survival rate[2] and dilatation functi…     

Measuring radial Young’s modulus of DNA by tapping mode AFM

Based on tapping mode AFM imaging, a method was demonstrated to evaluate compression elasticity of single double-stranded DNA （dsDNA） molecules in the force region. With images under ambient conditions, Young＇s moduli of dsDNA in compression were calculated. Results demonstrated that Young＇s moduli of dsDNA can be simply deduced according to the proposed model. The method can also be used to evaluate the compression elasticity of similar soft nanomaterials.     

Wavelet Numerical Solutions for Weakly Singular Fredholm Integral Equations of the Second Kind

Daubechies interval wavelet is used to solve numerically weakly singular Fredholm integral equations of the second kind. Utilizing the orthogonality of the wavelet basis,the integral equation is reduced into a linear system of equations. The vanishing moments of the wavelet make the wavelet coefficient matrices sparse,while the continuity of the derivative functions of basis overcomes naturally the singular problem of the integral solution. The uniform convergence of the approximate solution by the wavelet method is proved and the error bound is given. Finally,numerical example is presented to show the application of the wavelet method.     

基于Gauss滤波的多媒体图像的可视化检索

基于图像的可视化特征的图像检索和查询，是当前多媒体系统的重要研究方向。对于基于图像局部匹配的检索，传统的模板匹配和区域分割方法只能在目标图像中检索非常相似的对象，对尺度或形状有较大变化的目标则难以得到满意的结果。提出了一种不依赖于模板匹配的方法——图像的多尺度高斯滤波表示，用于图像的特征提取和局部匹配，并在分布并行索引结构下实现了该方法的快速索引和搜索算法。实验表明，该方法能够在较大尺度和视角变化范围内，更有效地进行图像的特征提取和检索，对基于图像局部区域相似性的匹配能达到较好的效果。     

热网监测系统的数据处理

鸡西市热力公司热网监测系统设计，数据处理采用上位机整点循环计算的方案，并将数据存入二进制文件中，收到较好的效果。     

医用历四柱干支显现的基本规律

本文依据天文万年历研究了医用历四柱干支显现的基本规律：（１）共有５１８４００种四柱干支组；历经一个６０甲子年显现的一半；历经２个６０甲子年显现的３／４；历经３个６０了年大多数显现；历经４个６０甲子年才全部显现。（２）四柱干支历经４个６０甲子年基本重现，医用历轮回周期的２４０年。     

复合式防水板在隧道防排水施工中的应用

随着我国既有线铁路大面积提速改造及快速铁路、高速铁路的修建,一些新技术、新材料、新工艺时于隧道工程的设计理论和施工技术带来了新的挑战,同时在许多方面深化和改革了传统的设计思路和施工方法.目前运营铁路中,隧道渗漏水已逐渐成为危及铁路行车安全的一大病害,隧道防排水工作已越来越受到建设、设计、施工、运营等单位的高度最视.文章着重介绍了隧道防排水施工中复合式防水板的应用及施工注意事项.     

碱提法回收猪骨蛋白质的探讨

对碱提法回收猪骨蛋白质工艺参数的研究表明，4种工艺参数对蛋白质得率均有不同程度的影响。其顺序为浸提液pH＞浸提时间＞沉降pH＞浸提温度。碱提法回收猪骨蛋白质的最高得率为25．7％，最佳工艺参数为浸提时间105min，浸提液pH12．0，浸提温度30℃和沉降pH5．0。     

Label-free selective sensing of Pb2+ lead(II) sensors based on the aggregation of a pyrene fluorescent probe

In the present work, we report a label-free fluorescence turn-on approach for the sensitive and selective sensing of Pb²⁺. Pyrene with one positive charge was used as the fluorescent probe, and thrombin aptamer (TBA), which was a G-rich oligonucleotide, was employed to form G-quadruplex with lead(II). When TBA and Pb²⁺ were mixed with lead(II) in an aqueous solution, it was folded into a stable G-quadruplex. Subsequently, a single-stranded nucleic acid-specific nuclease S1 was added. The G-quadruplex stabilized by Pb²⁺ lead(II) had markedly a significant resistant ability to nuclease S1 digestion. However, in the absence of Pb²⁺ lead(II), no quadruplex or less stable quadruplex was formed and TBA was digested by nuclease S1 in 3 min under the optimized experimental conditions. Finally, pyrene probe was mixed with oligonucleotide in Pb²⁺ lead(II). Electrostatic interactions between oligonucleotide (a polyanion) and the probe induced the aggregation of the probe, which in turn produced strong emission of the strong pyrene excimer emission. The intensity of the induced excimer emission was directly proportional to the amount of Pb²⁺ added. Our approach shows good selectivity and sensitivity for the detection of Pb²⁺ with a limit of detection limit as low as 800 nmol/L.     

复杂地区可控震源高效采集技术*

中国西部地区地表多以戈壁沙漠为主，非常适合可控震源施工，随着油气勘探和开发的不断深入，高密度、宽方位或全方位三维地震勘探技术被大量应用，随之带来巨大的成本压力。阐述了可控震源从传统的多台多次组合激发，逐步发展形成的拆分振次技术、交替扫描技术、滑动扫描技术、拆分台次技术、谐波压制技术。这些技术突破了可控震源传统的扫描方法，通过相互结合，联合应用，生产效率大大提高，单炮激发成本大幅降低，很好地解决了高密度地震勘探的成本问题，有效推动了西部复杂探区高密度、高覆盖、全方位三维地震勘探技术的推广应用。2005年以来，在对可控震源激发参数进行大量研究和试验基础上，逐步形成了可控震源经济、高效的激发技术和参数优化方法。在TH盆地致密砂岩油气藏勘探中，不仅中浅层信噪比明显提高，同相轴连续性明显增强，同时深层以及煤下和层间弱反射层的信噪比也有较大提高，进一步推动了西部盆地致密砂岩油气藏的勘探进程。